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論文中文名稱:洋蔥黑豆複方對大鼠去卵巢所誘發骨質疏鬆之改善作用 [以論文名稱查詢館藏系統]
論文英文名稱:Protective Effects of Onion Black Bean Herbal Formula Against Ovariectomized Osteoporosis in Rats [以論文名稱查詢館藏系統]
院校名稱:臺北科技大學
學院名稱:工程學院
系所名稱:生化與生醫工程研究所
畢業學年度:102
出版年度:103
中文姓名:王意菁
英文姓名:Yi-Ching Wang
研究生學號:100688011
學位類別:碩士
語文別:中文
口試日期:2014-01-14
論文頁數:39
指導教授中文名:黃志宏
口試委員中文名:張定國;侯劭毅
中文關鍵詞:骨質疏鬆症去卵巢手術
英文關鍵詞:OsteoporosisOvariectomized
論文中文摘要:骨質疏鬆症是一種骨骼老化的現象,它會造成骨質流失並使骨頭容易發生骨折。隨著國人平均壽命增加,骨鬆症已躍升至全球第二大流行病,僅次於心血管疾病,而其中占最大比例的患者,主要為停經後婦女,由於停經後婦女體內雌激素的急劇下降,使得骨質快速流失,而造成骨鬆症。目前臨床的藥物治療上,有可能會造成一些嚴重的副作用,如:大腿骨非典型骨折與下顎骨壞死等。故此實驗的目的,在於尋求一個安全性高的可替代療法,來延緩停經後骨質流失的情形。
此實驗將大鼠進行去卵巢手術,以模擬婦女停經後所造成骨質疏鬆症的動物模式,並給予洋蔥黑豆複方來探討對骨質疏鬆之改善作用。在大鼠進行去卵巢手術18周後,當達到骨質疏鬆模式後再給予洋蔥大豆複方做處置。發現給予複方13周後,可明顯地增加脊椎骨的骨密度;餵食15周後,股骨的骨小樑面積也明顯地提升。結果證實,給予洋蔥黑豆複方能有效地改善大鼠去卵巢後的骨質疏鬆情形。此複方未來極具潛力成為一個安全性高的食品補充劑,並可開發成改善骨密度或延緩婦女停經後所產生之骨質疏鬆症的健康食品。
論文英文摘要:Osteoporosis is a metabolic bone disease that causes the bone tissue to lose its normal structure and become fragile. It has risen to second pandemic disease in the world after cardiovascular disease. The majority of patients of osteoporosis were the post-menopausal women. It is mainly linked to the dramatic decrease in estrogens after menopause. Clinically, current osteoporosis medications may cause a variety of side effects, for example: atypical femoral fracture and osteonecrosis of the jaw. The aim of this study was to seek a natural product as an alternative treatment for management of postmenopausal osteoporosis.
Ovariectomized (OVX) rats are the most commonly animal model for studying osteoporosis in menopausal women. The rats were fed with the Onion Black Bean Herbal Formula after significant loss of lumbar BMD 18 weeks after surgery (OVX). Our data illustrated that the Formula not only had a significant preventive effect against bone loss in the lumbar spine after a-13week-feeding, but also significantly increase the trabecular area in the femur bone after a-15-week-feeding. In conclusion, the Onion Black Bean Herbal Formula may have a protective effect against ovariectomized osteoporosis in Rats. This formula could be a great potential as an alternative treatment and develop a healthy food for preventing osteoporosis in the future.
論文目次:目 錄

中文摘要 i
英文摘要 ii
誌謝 iv
目錄 v
圖目錄 vii
表目錄 viii
第一章 緒論 1
1.1節 研究背景 1
1.2節 骨骼的代謝 2
1.2.1節 骨骼組織 2
1.2.2節 骨骼恆定 2
1.2.3節 骨骼老化 3
1.3節 骨質疏鬆症 3
1.3.1節 骨質疏鬆症的成因 3
1.3.2節 骨質疏鬆症的分類 4
1.3.3節 質疏鬆症的併發症 4
1.4節 停經後骨質疏鬆症之預防與治療 5
1.4.1節 預防方法 5
1.4.2節 治療方法 6
1.5節 研究動機 7
1.6節 文獻回顧 7
1.6.1節 洋蔥 7
1.6.2節 黑豆 8
第二章 材料與方法 10
2.1節 實驗架構 10
2.2節 實驗材料 11
2.2.1節 去植物性雌激素配方 11
1.2.2節 洋蔥黑豆複方製備 11
2.3節 實驗儀器 12
1.3.1節 DEXA 12
1.3.2節 切片機 12
1.3.3節 Micro-CT 12
2.4節 實驗步驟 12
2.4.1節 卵巢摘除手術與藥物劑量分組 12
2.4.2節 樣本收集與處理 13
2.5節 分析方法 13
2.5.1節 骨密度檢測-腰部脊椎骨 13
2.5.2節 骨小樑面積測定-左股骨 14
2.5.3節 骨小樑微結構分析-腰部脊椎骨 14
2.5.4節 ANOVA分析法 15
第三章 結果 15
3.1節 骨礦物密度 15
3.2節 骨小樑面積 16
3.3節 骨小樑3D立體微結構 16
3.4節 體重變化 17
第四章 討論 18
第五章 結論與未來展望 21
5.1 結論 21
5.2 未來展望 21
參考文獻 23
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